NHA Certified Phlebotomy Technician (CPT)Collection RoutineEasy

A phlebotomy technician is preparing to collect a blood specimen for a prothrombin time (PT) test. Which of the following tubes should the technician use?

  1. ALavender top tube
  2. BLight blue top tube
  3. CRed top tube
  4. DGray top tube
Show answer & explanation

Correct answer: B. Light blue top tube

A prothrombin time (PT) test, which measures the time it takes for blood plasma to clot, requires a light blue top tube containing sodium citrate as an anticoagulant. Sodium citrate prevents clotting by binding calcium, preserving the coagulation factors for accurate testing.

Why the other options are wrong

  • A. Lavender top tubes contain EDTA and are used for hematology tests like CBC.
  • C. Red top tubes are plain tubes or contain clot activators and are used for serum chemistry or blood banking.
  • D. Gray top tubes contain sodium fluoride and potassium oxalate and are used for glucose and lactate testing.

Light Blue Top Tube

A vacuum blood collection tube identified by its light blue cap, containing sodium citrate as an anticoagulant.

  • Additive: Sodium Citrate.
  • Mechanism: Binds calcium to prevent clotting.
  • Primary Use: Coagulation studies (PT, PTT, INR).

Memory trick: PT/PTT: Light Blue for Coagulation's Clue.

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