NHA Certified Phlebotomy Technician (CPT)Collection RoutineMedium

A phlebotomy technician is instructing a patient on the proper collection of a 24-hour urine specimen. Which of the following instructions is essential for the patient to follow?

  1. ADrink at least 4 liters of water during the collection period.
  2. BStore the collection container at room temperature.
  3. CDiscard the first voided urine of the collection period.
  4. DCollect the first morning urine on the day the collection begins.
Show answer & explanation

Correct answer: C. Discard the first voided urine of the collection period.

To ensure an accurate 24-hour collection, the first voided urine of the collection period (e.g., first morning urine) should be discarded. This marks the *start* of the 24-hour period, and all subsequent urine for the next 24 hours, including the first void of the next morning, is collected.

Why the other options are wrong

  • A. Drinking excessive water can dilute the specimen and interfere with accurate measurement; normal hydration is usually recommended.
  • B. 24-hour urine specimens should typically be kept refrigerated or on ice to prevent degradation of analytes.
  • D. Collecting the first morning urine would make the collection period longer than 24 hours and is incorrect.

24-Hour Urine Collection

A method of collecting all urine produced over a full 24-hour period to measure the total amount of certain substances excreted by the kidneys, providing a comprehensive assessment of renal function.

  • Crucial for measuring substances like creatinine, protein, hormones.
  • Discard the first void; collect all subsequent urine for 24 hours.
  • Keep specimen refrigerated to preserve analytes.
  • Missed collections invalidate the entire sample.

Memory trick: 24-HOUR Rule: Hurl First, Observe All, Understand Timing, Refrigerate.

More Collection Routine questions