NHA Certified Phlebotomy Technician (CPT)Specimen HandlingMedium

A phlebotomy technician is preparing to transport a blood specimen that requires protection from light. Which of the following transport methods is most appropriate?

  1. ATransporting the specimen in a cooler with ice packs.
  2. BWrapping the specimen tube in aluminum foil or using an amber-colored tube.
  3. CPlacing the specimen in a clear plastic biohazard bag.
  4. DLeaving the specimen exposed on the phlebotomy tray during transport.
Show answer & explanation

Correct answer: B. Wrapping the specimen tube in aluminum foil or using an amber-colored tube.

Specimens sensitive to light, such as bilirubin or vitamin B12, must be protected from photodecomposition. Wrapping in aluminum foil or using an amber-colored tube effectively blocks light exposure.

Why the other options are wrong

  • A. Coolers with ice packs are for temperature control, not light protection.
  • C. Clear bags offer no light protection and are only for biohazard containment.
  • D. Exposure to light would degrade light-sensitive analytes, leading to inaccurate results.

Light-Sensitive Specimen Handling

Light-sensitive specimens, like bilirubin, must be protected from light exposure during collection, transport, and storage to prevent degradation of analytes and ensure accurate test results.

  • Use amber tubes or wrap in foil.
  • Minimize exposure time.
  • Common analytes include bilirubin, vitamin B12, folate.

Memory trick: Light is a Foe, Foil is a Shield.

More Specimen Handling questions